The female and egg of Anopheles dominicanus sp. n. are described from Dominican amber. The species is placed tentatively into the subgenus Nyssorhynchus on the basis of the following wing characters: a preapical pale costal spot; an accessory sector pale spot that is not joined to a sector pale spot; pale scales at the apex of vein R_{4 5}; basal, median, and apical pale spots on vein A; and the termination of vein Sc at the proximal end of the subcostal pale spot. This newly described mosquito from Dominican amber establishes an ancient lineage of Anopheles in the New World.